The inclusion bodies in the experiment were chunks in the cell; the cell had been altered by the Marburg virus. The cell was covered with pure crystal blocks of virus as found in Dan's experiment. (Preston, 1994). An inclusion body can be round or irregular shaped in the cytoplasm and nucleus and has to do with certain viral infections; it is usually made of protein (Inclusion body, n.d.). They are abnormal structures, and they are collections of maturing viruses or remains of virus replication. There are factors that can make inclusion bodies. For E. coli the fused protein A of Staphylococcus aureus and beta-galactosidase were examined. The induction temperature, PH in the cultivation medium, and amino acid sequence change changed the E- coli. The bodies and recombinant protein formed at 42 degrees Celsius. Twenty percent of the recombinant protein became part of the inclusion bodies and were insoluble. At 39 to 44 degrees Celsius, the protein became inclusion bodies. The conclusion was that PH was the control of the inclusion bodies, and more inclusion bodies were made as the PH decreased. The insoluble protein came from the soluble protein. Also, if the amino acid sequence in the linker region between the fusion protein were changed, then the inclusion bodies wouldn't form (Strandberg, Enfors, 1991).
Strandberg, L., & Enfors, S. O. (1991). Factors influencing inclusion body formation in the production of a fused protein in Escherichia coli. Applied and Environmental Microbiology, 57(6), 1669–1674.
Inclusion body. (n.d.). Retrieved May 17, 2015, from http://www.thefreedictionary.com/inclusion body
Inclusion bodies, as Helen says, are masses of viral particles found in cells. They can be either organic or inorganic. Organic inclusion bodies are inclusion bodies composed of cyanophycean starch granules and glycogen granules. Inorganic inclusion bodies are composed of polyphosphate granules and sulphur granules. Organic inclusion bodies are often used as storage vessels, specifically nitrogen in cyanobacteria. Inorganic inclusion bodies are used as storage vessels as well, but for phosphate and sulfur. Inclusion bodies are characteristic feature of the Ebola virus in cells. Despite the fact that they contain unusually large count of performed nucleocaspids, their biological significance was unknown. Recent theorize that inclusion bodies are involved in viral “genome replication and transcription” (Virol, 2012).
The inclusion bodies in the experiment were chunks in the cell; the cell had been altered by the Marburg virus. The cell was covered with pure crystal blocks of virus as found in Dan's experiment. (Preston, 1994). An inclusion body can be round or irregular shaped in the cytoplasm and nucleus and has to do with certain viral infections; it is usually made of protein (Inclusion body, n.d.). They are abnormal structures, and they are collections of maturing viruses or remains of virus replication. There are factors that can make inclusion bodies. For E. coli the fused protein A of Staphylococcus aureus and beta-galactosidase were examined. The induction temperature, PH in the cultivation medium, and amino acid sequence change changed the E- coli. The bodies and recombinant protein formed at 42 degrees Celsius. Twenty percent of the recombinant protein became part of the inclusion bodies and were insoluble. At 39 to 44 degrees Celsius, the protein became inclusion bodies. The conclusion was that PH was the control of the inclusion bodies, and more inclusion bodies were made as the PH decreased. The insoluble protein came from the soluble protein. Also, if the amino acid sequence in the linker region between the fusion protein were changed, then the inclusion bodies wouldn't form (Strandberg, Enfors, 1991).
ReplyDeleteStrandberg, L., & Enfors, S. O. (1991). Factors influencing inclusion body formation in the production of a fused protein in Escherichia coli. Applied and Environmental Microbiology, 57(6), 1669–1674.
Inclusion body. (n.d.). Retrieved May 17, 2015, from http://www.thefreedictionary.com/inclusion body
ReplyDeleteInclusion bodies, as Helen says, are masses of viral particles found in cells. They can be either organic or inorganic. Organic inclusion bodies are inclusion bodies composed of cyanophycean starch granules and glycogen granules. Inorganic inclusion bodies are composed of polyphosphate granules and sulphur granules. Organic inclusion bodies are often used as storage vessels, specifically nitrogen in cyanobacteria. Inorganic inclusion bodies are used as storage vessels as well, but for phosphate and sulfur. Inclusion bodies are characteristic feature of the Ebola virus in cells. Despite the fact that they contain unusually large count of performed nucleocaspids, their biological significance was unknown. Recent theorize that inclusion bodies are involved in viral “genome replication and transcription” (Virol, 2012).